1016. Modulation of Transgene Expression: DNA Methylation Is, at Best, Only Partly Responsible
نویسندگان
چکیده
منابع مشابه
Modulation of plasmid DNA methylation and expression in zebrafish embryos.
Gene expression is under the influence of DNA methylation and assembly of chromatin structure. This paper reports the modulation of transgene expression in zebrafish embryos by altering DNA methylation with 5-azacytidine and heterochromatin formation with sodium butyrate, an inhibitor of histone deacetylation. A CMV promoter-luciferase fusion gene construct (pCMVL) microinjected into zebrafish ...
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The splendid colours and complex patterns of the plumage, eggs, and beaks, feet and other bare parts of birds have captivated humans for millenia and scientists for more than 150 years. In particular, birds have provided a rich basis for mechanistic studies of feather structure and plumage development, predominant through much of the 20th century, and for evolutionary studies of functional aspe...
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Recombinant adeno-associated viral (rAAV) vectors can support long-term transgene expression in quiescent tissues. Intramuscular (i.m.) administration of a single-stranded AAV vector (ssAAV) in the nonhuman primate (NHP) results in a peak protein level at 2-3 months, followed by a decrease over several months before reaching a steady-state. To investigate transgene expression and vector genome ...
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BACKGROUND Integrating non-viral vectors based on transposable elements are widely used for genetically engineering mammalian cells in functional genomics and therapeutic gene transfer. For the Sleeping Beauty (SB) transposase system it was demonstrated that convergent transcription driven by the SB transposase inverted repeats (IRs) in eukaryotic cells occurs after somatic integration. This co...
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ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2006
ISSN: 1525-0016
DOI: 10.1016/j.ymthe.2006.08.1110